Plant Tissue Culture Laboratory (PTCL) Bangalore

PTCL Bangalore

Achievements

With a highly qualified set of staff, dedicated researchers and Inspire Scholarship holding students working in the plant tissue culture laboratory, it is not surprising to see that along with the work done; even the knowledge of the people in the laboratory is gradually growing. There is some new finding or deeper understanding in the field of plant tissue culture almost every day. People working and learning in this laboratory are evolving by the day.

Some of the noteworthy findings of this laboratory are:


  • National award winning break-through in cost reduction of tissue cultured Banana plantlets by utilization of alternative carbon sources, gelling agents, matrices, water sources and light.
  • Similar achievements in production of Potato and Philodendron plantlets.
  • First report on callus induction in Ashoka (Saraca indica)
  • Reported occurrence of somatic embryogenesis in sweet type carambola, for the first time
  • First successful attempt in obtaining in vitro shoot proliferation in solid bamboo
  • In vitro colchicine production from callus cultures of Gloriosa superba, an important medicinal plant was reported for the first time.


The plant tissue culture laboratory has also developed several protocols which can be used by the production unit of the laboratory and also production units of other corporate companies. Some of the protocols are:

POMOLOGY / FRUIT SCIENCE

  • Development of a nationally acknowledged low cost micropropagation protocol for the leading banana Cv. ‘Grand Naine’. Our comprehensive studies have shown the possibility of reducing the cost of plant production in the laboratory by 20-30%.
  • In Nanjangud Rasabale,a threatened premier variety of Banana of Karnataka with a G I tag, an ambitious project is underway to increase the frequency of plant production (disease free) through micropropagation, and also to intensify the work on good cultivation practices. The work is also to identify naturally occurring variants. A protocol for mass multiplication of Banana Cv. Dwarf Cavendish, Robusta, Nendran (Red Banana) and Grand Naine was developed earlier. An estimation of yield potentialities of these tissue-cultured plants has also been done.
  • Extensive studies on micropropagation of a popular grape Cv. ‘Gulabi’, an interesting variety of grapes. Though this has been a revered variety for its impeccable rose flavor, suffers from uneven ripening problem. Hopefully our work would pave for its renewed cultivation in Karnataka. We have been successful in developing protocol for micropropagation of Gulabi as well as Thompson Seedless a popular commercial variety.
  • In Jackfruit elite cultivar Singapore jack, a protocol for micropropagation was developed.
  • In Strawberry, a protocol for mass multiplication of cultivars Sujatha and Labella was developed.
  • A good protocol has been developed for direct regeneration of plants from nodal segments of Tahiti Lime (Citrus latifolia).
  • In sweet type Star fruit (Carambola), for the first time occurrence of somatic embryogenesis was reported & a protocol for direct and indirect somatic embryogenesis has been developed.

OLERICULTURE / VEGETABLE SCIENCE

  • In Potato, a protocol for In vitro tuberisation and micropropagation has been developed. A noteworthy achievement in potato has been the use of Tapioca as a gelling agent instead of agar. Tapioca source is found to be a cheapest source, thus facilitating a substantial decrease in the cost of tissue culture production of plants.
  • In Curry leaf a protocol was developed for micropropagation
  • In Chilli, exhaustive work was done to study callusing. The work has a bearing in future research towards varietal development in vitro. The varieties used for the study include famous Byadagi dabba and Arka series.

FLORICULTURE AND ORNAMENTALS

  • Successful low cost micropropagation of Philodendron spp. Var. ‘Xanadu’.
  • In Tinospora, a protocol for callus induction and micropropagation was standardized.
  • In Anthurium Cv. Ceylon Crinkle Red, a regeneration protocol was developed and a protocol for seed germination was also developed.
  • In Carnation, in an exhaustive study, various methods of direct and indirect regeneration protocol were developed. A varietal evaluation in regard to response to callusing was studied in detail.
  • In Dendrobium Orchid, Cv. “Jo Mutant”, a protocol for in vitro proliferation was developed. A high frequency proliferation has been achieved. This work has a commercial bearing.
  • In Solid Bamboo (Oxytenenthera stocksii) for the first time, in vitro shoot proliferation has been reported.
  • Induction of callus from matured leaves and petiole segments in Jasmine

MEDICINAL AND AROMATICS

  • In Long pepper, a protocol for micropropagation was standardized.
  • In Coleus, a protocol for micropropagation, callusing, somatic embryogenesis was developed. This has a bearing on the further crop improvement programme in this crop.
  • In Patchouli, a protocol for callusing and a very high frequency shoot proliferation has been standardized.
  • Brahmi (Centella asiatica): Standardization of protocol for direct and indirect regeneration from different explants.
  • Standardization of protocol for direct and indirect regeneration in Safed Musli has been standardized.
  • In Qat (Catha edulis) , an exciting medicinal crop of Yemen, work on tissue culture and a detailed study on varietal distribution and variance through genetic markers has been successfully completed. This work ,in our opinion, is the most comprehensive study to-date. It assumes significance from the fact that collection and evaluation of plant material representing various geographical locations across Yemen.
  • First report on callus induction in Ashoka (Saraca indica)
  • In vitro studies on regeneration and acclimatization in Stevia (Stevia rebaudiana Bert.), a problem of reduced success rate during hardening was addressed and we were able to achieve 70-80% success through our research on establishment through treatments both in vitro and in vivo stages.
  • In vitro colchicine production from callus cultures of Gloriosa superba, an important medicinal plant was reported for the first time, as well as a protocol for micropropagation was developed.

NOTE :
The related full length research papers authored by Dr.B.N.Sathyanarayana and faculty can be downloaded through www.google.com , by the search names B.N.Sathyanarayana / Dr.B.N.Sathyanarayana.

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  • Banana
  • Phyllodendron Xanadu
  • Brahmi
  • Syngonium
  • Stevia
  • Ginger
  • Patchouli
  • Grapes
  • Anthurium
  • Orchids

 
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